Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,778,380	G→A	T360T (ACG→ACA)	ydiF →	fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,778,380	0	G	A	100.0%	11.6 / NA	6	T360T (ACG→ACA)	ydiF	fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/0);  total (6/0)

CCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTT  >  W3110S.gb/1778359‑1778432                      |                                                     ccGTGCCATTCTGGATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGAtgttt              >  1:2487283/1‑62 (MQ=255)             ggctatGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt  >  1:1647162/4‑62 (MQ=255)             ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt  >  1:1200694/1‑62 (MQ=255)             ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt  >  1:1375481/1‑62 (MQ=255)             ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt  >  1:2811795/1‑62 (MQ=255)             ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt  >  1:2869852/1‑62 (MQ=255)                      |                                                     CCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTT  >  W3110S.gb/1778359‑1778432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑