Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,819,760	G→T	A152E (GCG→GAG)	chbF ←	cryptic phospho‑beta‑glucosidase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,819,760	0	G	T	100.0%	37.9 / NA	13	A152E (GCG→GAG)	chbF	cryptic phospho‑beta‑glucosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/0);  total (13/0)

GACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATT  >  W3110S.gb/1819731‑1819791                              |                                gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCa            >  1:835774/1‑51 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:1112434/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:1399411/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:1739865/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:1806722/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:194547/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:1955816/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:2201164/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:2707162/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:2835421/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:2855988/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:32918/1‑61 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCAtt  >  1:435236/1‑61 (MQ=255)                              |                                GACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATT  >  W3110S.gb/1819731‑1819791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑