Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,825,900 G→A V224V (GTG→GTA)  ydjQ → endonuclease of nucleotide excision repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,825,9000GA84.9% 69.9 / 6.2 33V224V (GTG→GTA) ydjQendonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base A (28/0);  total (33/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

TCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAA  >  W3110S.gb/1825855‑1825949
                                             |                                                 
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAg                                         >  1:44943/1‑56 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2532305/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:893980/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:685588/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:655527/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:513481/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:421907/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:344320/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:317833/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2814526/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2762811/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2621590/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1144126/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2451115/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1509155/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1220377/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1267444/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1267866/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1312947/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1336490/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2415420/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1646995/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1862370/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:1946317/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2042133/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2226879/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCTGAAAGAACAGc                                   >  1:2401249/1‑62 (MQ=255)
tctGGAACGTTTACGAGTCGTTTGTTGGCCGTGGAAAGGTGCGGTAGCGCAGAAAGAACAGc                                   >  1:673550/1‑62 (MQ=255)
                                 gCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCaaa  >  1:2909619/1‑62 (MQ=255)
                                 gCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCaaa  >  1:382974/1‑62 (MQ=255)
                                 gCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCaaa  >  1:1290286/1‑62 (MQ=255)
                                 gCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCaaa  >  1:2574509/1‑62 (MQ=255)
                                 gCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCaaa  >  1:858354/1‑62 (MQ=255)
                                             |                                                 
TCTGGAGCGTTTGCGGGTGGTGTGTTGGCCTTGGCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAAA  >  W3110S.gb/1825855‑1825949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑