| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,842,719 | T→C | A133A (GCA→GCG) | ynjF ← | predicted phosphatidyl transferase, inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,842,719 | 0 | T | C | 76.7% | 75.2 / 19.7 | 43 | A133A (GCA→GCG) | ynjF | predicted phosphatidyl transferase, inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (10/0); new base C (0/33); total (10/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
AAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACA > W3110S.gb/1842673‑1842774 | aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:414930/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2263935/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1067332/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2495285/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2522104/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2651462/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2665161/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2802340/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2882137/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:377002/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2296859/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:509305/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:550325/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:614446/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:665667/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:887119/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:915861/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:962792/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:978248/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1738041/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1082057/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1184289/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1229842/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1235477/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1535216/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1552446/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2254759/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1756840/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1947998/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2135986/62‑2 (MQ=255)aaaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2171842/62‑2 (MQ=255) aaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:2751992/61‑2 (MQ=255) aaaCGATTTATGCGCATAACCGGGATTATCAATCTGATGTTTCGCCGCCAGCGCAGCaaaa < 1:1912078/61‑2 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:2721046/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:1685388/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:2008024/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:2520428/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:1512823/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:1439973/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:616534/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:124931/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:2434966/1‑61 (MQ=255) tttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaaca > 1:2145513/1‑61 (MQ=255) | AAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACA > W3110S.gb/1842673‑1842774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |