Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,866,607	A→G	H248H (CAT→CAC)	yeaE ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,866,607	0	A	G	100.0%	67.5 / NA	21	H248H (CAT→CAC)	yeaE	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/21);  total (0/21)

AAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAA  >  W3110S.gb/1866570‑1866632                                      |                          aaaGTTCGACCTCAAGCACAGCCGCA‑TTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:289715/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2652051/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:845825/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:810290/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:652642/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:606236/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:522045/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:512345/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:49909/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2745358/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2726989/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:1434490/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2630903/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2512088/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2259953/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2201308/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:1925270/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:1633291/62‑1 (MQ=255)  aaGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:1594431/62‑1 (MQ=255)               aGCACAGCCGCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:1542424/49‑1 (MQ=255)                        gCATTTTGTTGGACGTGGGCAATCGTGGCCGCTTTTGGaa  <  1:2327606/40‑1 (MQ=255)                                      |                          AAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAA  >  W3110S.gb/1866570‑1866632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑