BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,871,237	C→T	A190V (GCA→GTA)	yeaH →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,871,237	0	C	T	100.0%	184.5 / NA	56	A190V (GCA→GTA)	yeaH	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/48); total (8/48)

ACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAG  >  W3110S.gb/1871200‑1871293

acaGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCcatc                                    >  1:1301616/1‑60 (MQ=255)

acaGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCcatc                                    >  1:1321544/1‑60 (MQ=255)

          cGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACa                          >  1:2436920/1‑60 (MQ=255)

          cGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACa                          >  1:2151427/1‑60 (MQ=255)

          cGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAg                         >  1:143298/1‑61 (MQ=255)

          cGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAg                         >  1:1510295/1‑61 (MQ=255)

          cGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAg                         >  1:778603/1‑61 (MQ=255)

          cGGCAGGCAAGCGGCGGGAACTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAg                         >  1:1908180/1‑61 (MQ=255)

                            aaCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:2673257/62‑1 (MQ=255)

                            aaCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:2302560/62‑1 (MQ=255)

                            aaCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:2875480/62‑1 (MQ=255)

                            aaCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:751767/62‑1 (MQ=255)

                            aaCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:1722526/62‑1 (MQ=255)

                            aaCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:1661800/62‑1 (MQ=255)

                             aCTTCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgctg      <  1:2626192/61‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTTCGCAACTGCTGGAag  <  1:1762551/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:522756/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2733557/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2628381/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2807768/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2840595/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2600497/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:310136/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:335778/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:422873/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:507005/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:517585/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2401298/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:85320/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:862713/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:869909/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:909326/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:916955/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2222743/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1138295/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1236078/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1354701/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1460880/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1723807/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1958871/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:197982/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2012906/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2219094/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2219463/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2563678/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2236978/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2239286/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2342769/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2353416/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2372201/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2383379/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:1081819/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2408630/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2431358/62‑1 (MQ=255)

                                tCATGTACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:2542272/62‑1 (MQ=255)

                                tCATGTAATGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag  <  1:699603/62‑1 (MQ=255)

ACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAG  >  W3110S.gb/1871200‑1871293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`