Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,872,347	A→G	K83K (AAA→AAG)	yeaI →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,872,347	0	A	G	100.0%	24.5 / NA	9	K83K (AAA→AAG)	yeaI	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/0);  total (9/0)

CACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTC  >  W3110S.gb/1872335‑1872395             |                                                 gacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:1647227/2‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:1028428/1‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:1448280/1‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:1550848/1‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:2261029/1‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:2687847/1‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:277184/1‑61 (MQ=255) cacaAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc  >  1:554475/1‑61 (MQ=255) cacaAATGTAAAGCTAATCCCACAGATGAATTACCTGATGgttgtt                 >  1:10844/1‑46 (MQ=255)             |                                                 CACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTC  >  W3110S.gb/1872335‑1872395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑