Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,962,789	C→T	D338D (GAC→GAT)	argS →	arginyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,962,789	0	C	T	83.5%	202.9 / 29.3	91	D338D (GAC→GAT)	argS	arginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (14/1);  new base T (0/76);  total (14/77)
Fisher's exact test for biased strand distribution p-value = 1.40e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTC  >  W3110S.gb/1962737‑1962831                                                     |                                           cGAAATATCGTTATGAAAGACTGCATGCCGATCGCGTGCGGTATTACATCGATTCCCGTc                                     <  1:2421918/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGTCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1444731/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2496993/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2693975/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2691026/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2674552/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2645238/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2577692/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:255284/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:252833/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2500707/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2725911/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:549017/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2417710/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:238364/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2347966/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:635018/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2302229/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2239175/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:22274/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:324489/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:491027/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:478058/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:447480/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:438031/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:419672/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:492125/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:342990/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:342934/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:220121/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2915345/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2914546/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2895208/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:289050/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2836223/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:540660/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2793952/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2774941/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1349245/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1562443/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:839635/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:841030/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1513889/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1486164/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1440107/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1421425/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1390926/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1701208/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1330574/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1316552/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1297988/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:868132/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1244190/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1181558/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:908169/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1074259/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1851049/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2056492/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2047721/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:2041071/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:672186/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:695499/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1967127/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1894005/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1869717/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1831920/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:724315/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1803842/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:778277/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1735537/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1721462/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1705998/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1702746/60‑1 (MQ=255) cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTc                                     <  1:921662/60‑1 (MQ=255)  gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:1746824/59‑1 (MQ=255)        tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:386017/53‑1 (MQ=255)                     cTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc                                     <  1:115882/40‑1 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCa                  >  1:1969903/1‑45 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATTGGCGATCGTc  >  1:1124700/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGt   >  1:801186/1‑60 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:2023878/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:2323872/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:722983/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:1807355/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:2446649/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:1560152/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:15400/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:1265162/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:896993/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:2814340/1‑61 (MQ=255)                                   cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc  >  1:486951/1‑61 (MQ=255)                                                     |                                           CGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTC  >  W3110S.gb/1962737‑1962831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑