Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,985,959	T→C	E416E (GAA→GAG)	araG ←	fused L‑arabinose transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,985,959	0	T	C	100.0%	28.3 / NA	10	E416E (GAA→GAG)	araG	fused L‑arabinose transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/0);  total (10/0)

TAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGAT  >  W3110S.gb/1985904‑1985965                                                        |       tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:1057395/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:1064644/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:1218974/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:1220489/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:1259481/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:1400948/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:151744/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:2581124/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:343946/1‑62 (MQ=255) tAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCCTCCGAt  >  1:526766/1‑62 (MQ=255)                                                        |       TAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGAT  >  W3110S.gb/1985904‑1985965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑