Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,012,333	A→G	I126V (ATT→GTT)	yedK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,012,333	0	A	G	100.0%	49.3 / NA	16	I126V (ATT→GTT)	yedK	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/0);  total (16/0)

GACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTT  >  W3110S.gb/2012288‑2012394                                              |                                                              gACAAAAAACAGCCTTATTTTATCTATCGCGGTGATGGACAACCTGTTTTTa                                                         >  1:1731900/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:1018482/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:306260/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:305412/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:2821074/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:2639512/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:253275/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:2417712/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:714513/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:2107696/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:787403/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:841562/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:159669/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:158771/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:841975/1‑52 (MQ=255) gACAAAAAACAGCCTTATTTTATCTATCGCGCTGATGGACAACCTGTTTTTa                                                         >  1:1362553/1‑52 (MQ=255)                                              aTTTTTATGGTCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:255411/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:785857/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:776055/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:696446/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:606500/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:909907/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:95555/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:332086/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2853302/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2807989/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2286336/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2261485/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2233337/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2096641/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:2061116/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:1537200/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:1323619/62‑1 (MQ=255)                                              aTTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGAttttt  <  1:1111658/62‑1 (MQ=255)                                              |                                                              GACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTT  >  W3110S.gb/2012288‑2012394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑