Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,060,763 A→C L407L (CTT→CTG yeeO ← predicted multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,060,7630AC79.1% 221.7 / 41.1 92L407L (CTT→CTGyeeOpredicted multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (4/15);  new base C (72/0);  total (77/15)
Fisher's exact test for biased strand distribution p-value = 7.07e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

AAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGC  >  W3110S.gb/2060718‑2060808
                                             |                                             
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:2158489/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:2864633/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:2900015/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:2927564/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:364665/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:1930143/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:276205/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:1656761/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:582319/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:207370/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:2609336/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:1083855/62‑1 (MQ=255)
aaaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:2495698/62‑1 (MQ=255)
 aaGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:1276212/61‑1 (MQ=255)
  aGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                               <  1:79131/60‑1 (MQ=255)
   gggAGCCGTTAGCCAGGCGCTGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:66424/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGc                                            >  1:676209/1‑46 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAg                                         >  1:1778398/1‑49 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGaa                                       >  1:242348/1‑51 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCgga                            >  1:553119/1‑60 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGc                             >  1:1694722/1‑61 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2421779/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:254836/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2857522/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2462972/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2489920/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2763558/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2491156/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2674476/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:463499/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2669038/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2504868/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:740229/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:994254/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:964693/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:946454/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:935871/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:903009/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:886378/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:783816/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:741386/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:295959/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:70899/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:648524/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:5978/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:512581/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:508792/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2359332/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:367870/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1537596/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1743561/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2415255/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1734244/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1714475/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1677648/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1597681/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1577875/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1567210/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1567133/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1736075/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1534135/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1422365/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1260946/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1235634/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1234564/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1197390/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1138542/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1088010/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1083228/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1983353/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2398183/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1037083/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2275923/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2268505/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2263919/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2246394/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2223795/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:220400/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2057340/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1813430/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:192519/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1910181/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1881727/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1834019/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGG‑‑AGCCAGAACACATGCCGCaa                           >  1:981911/1‑61 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAATCAAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:2163455/1‑62 (MQ=255)
   gggAGCCGTTAGCCAGGCGATGGCCGTTAACCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1641652/1‑62 (MQ=255)
   gggAGCCGTTAACCAGGCGATGGCCGTTAATCCAAGAGTGGACAGCCAGAACACATGCCGCa                            >  1:1950415/1‑62 (MQ=255)
                                         tGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGc  >  1:1601606/1‑50 (MQ=255)
                                         tGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGc  >  1:287838/1‑50 (MQ=255)
                                         tGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGc  >  1:527535/1‑50 (MQ=255)
                                         tGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGc  >  1:1895956/1‑50 (MQ=255)
                                             |                                             
AAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGC  >  W3110S.gb/2060718‑2060808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: