| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,066,123 | T→C | Q161R (CAG→CGG) | cobT ← | nicotinate‑nucleotide dimethylbenzimidazole‑P phophoribosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,066,123 | 0 | T | C | 90.5% | 234.1 / 11.0 | 85 | Q161R (CAG→CGG) | cobT | nicotinate‑nucleotide dimethylbenzimidazole‑P phophoribosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base C (76/0); total (77/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGTAT > W3110S.gb/2066066‑2066129 | tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:1493386/62‑1 (MQ=255)tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:356063/62‑1 (MQ=255)tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:2043560/62‑1 (MQ=255)tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:2700160/62‑1 (MQ=255)tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:1424854/62‑1 (MQ=255)tttGCCATCCCCAGTTCACCTACACCAAACAGCGGGACACCGTTTTTTGCCAGCTCCTGCGt < 1:2735413/62‑1 (MQ=255) ttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:135978/61‑1 (MQ=255) ttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:1481553/61‑1 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2491921/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2490938/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:333483/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:317495/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2876933/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2851619/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2851025/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2748318/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2736240/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2670099/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2616627/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2717693/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2561215/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:490880/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2675470/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2623759/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2575207/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:634888/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:961039/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:881997/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:796818/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:758441/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:735047/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:727396/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:72227/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:719411/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:637674/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:37825/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:628966/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:625307/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:604252/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:596199/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:584555/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:574116/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:521995/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2310922/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:463631/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1602985/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1755908/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2388318/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1749053/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1744406/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1732899/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1724385/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1650646/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:161596/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1603605/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1752952/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1598590/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:150839/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:141634/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1408176/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1215083/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1094202/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1091027/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1087969/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2084980/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2340050/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2317011/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1026078/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2302448/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2295774/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2266650/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2214773/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1818521/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2074901/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2048949/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2025625/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1965940/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1962917/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1877108/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1870377/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1845359/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGCCAGCTCCAGCGtat > 1:1741235/1‑36 (MQ=255) aCAGCGTGACACCGTTTTTTGACAGCTCCCGCGtat > 1:120643/1‑36 (MQ=37) aCAGCGTGACACCGTTTTATGCCAGCTCCCGCGtat > 1:1486192/1‑36 (MQ=37) aCAGCGTCACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2307615/1‑36 (MQ=37) | TTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGTAT > W3110S.gb/2066066‑2066129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |