Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,115,934	G→A	I177I (ATC→ATT)	galF ←	predicted subunit with GalU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,115,934	0	G	A	100.0%	114.4 / NA	35	I177I (ATC→ATT)	galF	predicted subunit with GalU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/35);  total (0/35)

AGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTG  >  W3110S.gb/2115928‑2115988       |                                                       ggTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:264386/60‑1 (MQ=255)  ggcTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2839664/58‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:289564/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2341653/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2431847/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2471873/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2474849/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2565928/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2690079/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2261638/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2922564/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:457324/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:461633/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:517441/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:608906/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:738765/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:879970/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:885320/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1716756/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1101385/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:114988/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1155022/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1388052/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1414116/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1633888/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1645096/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1022071/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1877368/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2040471/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2085573/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2188187/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2209140/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2222910/60‑1 (MQ=255)  gTCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:2233385/60‑1 (MQ=255)   tCTGAATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTg  <  1:1447457/59‑1 (MQ=255)       |                                                       AGTCTGGATGACGGAGTATTCAGAGAGGTCACCCGGCATACGTTTTGCCAGCACCTGGCTG  >  W3110S.gb/2115928‑2115988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑