Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,118,899 G→A F122F (TTC→TTT)  wcaL ← predicted glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,118,8990GA100.0% 313.4 / NA 108F122F (TTC→TTT) wcaLpredicted glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (108/0);  total (108/0)

Only 100 of 108 total aligned reads displayed.

CAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGG  >  W3110S.gb/2118848‑2118909
                                                   |          
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATc    >  1:1198162/1‑60 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATc    >  1:570492/1‑60 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:246144/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2899332/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2870053/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2826026/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2825899/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2776017/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2773014/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2746650/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2735621/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2731926/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2695530/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2592414/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:313342/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2443908/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2437523/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:240135/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:235100/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2349285/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2329511/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2292840/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2285239/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1032882/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2210735/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:606726/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:896684/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:865345/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:846274/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:821415/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:810667/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:763449/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:745915/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:73464/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:705266/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:662153/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:647844/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2901283/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:570942/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:567811/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:537404/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:491134/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:472324/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:437472/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:42125/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:347880/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:343727/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2263814/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1424336/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1603597/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2159207/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:153035/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1520184/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1510919/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1480127/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1467980/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1454827/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1442594/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1431464/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1576110/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1387984/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1382174/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1380012/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1378105/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1304149/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1232397/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1188629/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1163649/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1132688/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1087286/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1870749/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2146894/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2117471/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2103823/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:209795/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2095441/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2078726/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2024888/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1949112/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1900797/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1630415/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1833232/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1810976/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1777929/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1703445/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1694089/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:16854/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1686544/1‑62 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg   >  1:1766630/1‑61 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg   >  1:2140482/1‑61 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg   >  1:1585935/1‑61 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg   >  1:1192276/1‑61 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg   >  1:366120/1‑61 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCg   >  1:383976/1‑61 (MQ=25)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACAAATCgg  >  1:1539990/1‑62 (MQ=21)
cAGTTCGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCAAAAATGAGCGATAAACACATCgg  >  1:1553949/1‑62 (MQ=255)
cAGTTCGCGTAGTTTTGCCGCGGTTACCACAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:774940/1‑62 (MQ=21)
cAGTTCGCGTAGTTTTGCCGCGGTTACACCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2371527/1‑62 (MQ=21)
cAGTTCGCGTAGTTTTGCAGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:2438659/1‑62 (MQ=25)
cAGTTAGCGTAGTTTTGCCGCGGTTACCCCAGCAGGGCCAAAATGAGCGATAAACACATCgg  >  1:1601514/1‑62 (MQ=21)
                                                   |          
CAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGG  >  W3110S.gb/2118848‑2118909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑