Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,143,270	C→A	Q160H (CAG→CAT)	asmA ←	predicted assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,143,270	0	C	A	100.0%	330.4 / NA	98	Q160H (CAG→CAT)	asmA	predicted assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (78/20);  total (78/20)

CATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAA  >  W3110S.gb/2143222‑2143301                                                 |                                cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCTTGATGGAACACCAg                      >  1:1797364/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCa                       >  1:2321826/1‑59 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1810964/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:613723/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:65049/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:167011/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:178325/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2274844/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:715630/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2271562/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1591347/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1911898/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2200789/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2019631/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2022386/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2197958/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2036587/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2164563/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2039747/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:130544/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1120499/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:429759/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1168513/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:41944/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:544208/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1254830/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2918500/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1278305/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:544214/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2700562/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1362106/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:860965/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:5934/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1437551/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:2580224/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:791749/1‑60 (MQ=255) cATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAg                      >  1:1589513/1‑60 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTg               >  1:44929/1‑61 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTg               >  1:2261990/1‑61 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTg               >  1:1010699/1‑61 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTg               >  1:1485063/1‑61 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:933907/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2634076/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2638396/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2650576/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1419628/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:892138/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:271060/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2751781/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2757895/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:583242/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:21195/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2784724/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2815590/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2839904/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2868254/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1256842/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1246722/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:455069/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1145297/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:671887/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2037514/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2185770/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2030430/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:210598/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1998498/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2214008/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2248472/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1815693/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1801043/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:736003/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2296402/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1756629/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1629873/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2385992/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:631862/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:1591690/1‑62 (MQ=255)       cAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGt              >  1:2628515/1‑62 (MQ=255)                 ttGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCtt    <  1:2604198/62‑1 (MQ=255)                 ttGCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCtt    <  1:305/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:979783/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:2273904/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:1213566/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:130740/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:13541/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:136243/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:1786623/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:1790109/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:414748/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:2381368/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:2469363/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:268649/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:278201/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:320651/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTCATGATGGAACAACAGCACGCTGTCCGCCACCTTaa  <  1:254712/62‑1 (MQ=255)                   gCGGATTGTCACCTGCTCGTCATCTTAATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:277893/62‑1 (MQ=255)                     ggATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:1528828/60‑1 (MQ=255)                     ggATTGTCACCTGCTCGTCATCTTCATGATGGAACACCAGCACGCTGTCCGCCACCTTaa  <  1:897484/60‑1 (MQ=255)                                                 |                                CATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAA  >  W3110S.gb/2143222‑2143301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑