| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,146,254 | C→G | T284T (ACC→ACG) | yegE → | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,146,254 | 0 | C | G | 94.7% | 375.6 / 7.7 | 114 | T284T (ACC→ACG) | yegE | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base G (108/0); total (108/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
ATGACCATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAACCCA > W3110S.gb/2146249‑2146310 | atGACCATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAACCCa < 1:1617709/62‑1 (MQ=255)atGACCATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAACCCa < 1:2580659/62‑1 (MQ=255)atGACCATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAACCCa < 1:2291795/62‑1 (MQ=255)atGACCATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAACCCa < 1:2414699/62‑1 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCa > 1:151295/1‑35 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCa > 1:2576299/1‑35 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGaa > 1:441530/1‑55 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2608446/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2211129/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2766138/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2702215/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2269350/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2673232/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:265589/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2622179/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2617690/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2326310/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2602475/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2590006/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1004227/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2580488/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2287540/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2496914/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2478335/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2323699/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:49413/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:967201/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:934336/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:884612/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:881361/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:798939/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:723839/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:676465/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:555597/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:550465/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:547243/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:528670/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:278055/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:470491/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:462222/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:45905/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:368004/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:341252/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:319441/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:301008/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:291418/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2881753/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2841394/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1274399/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1519086/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2208068/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1487264/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1459786/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1442838/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1432900/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:142834/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1332632/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1326594/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1320497/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1279082/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1497676/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1270225/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1230112/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:118177/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1168211/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:110648/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1101202/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1094367/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1088072/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1061967/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1044140/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1008850/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1611219/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2177060/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2157387/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2149529/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:212139/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2120565/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2053272/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2043815/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:2020765/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:199013/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1969411/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1960734/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1561770/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1669982/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1704970/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:170761/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1839552/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:186752/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1868912/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAccc > 1:1908352/1‑59 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcc > 1:562149/1‑58 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcc > 1:569011/1‑58 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcc > 1:2530405/1‑58 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcc > 1:2680332/1‑58 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcc > 1:1300900/1‑58 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcac > 1:2555553/1‑57 (MQ=255) gACGATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAAcac > 1:886450/1‑57 (MQ=255) gACGATGGTGATGTATGCATTTCGTGCGGAACGCAAACACATTTCCGaa > 1:1976478/1‑49 (MQ=255) | ATGACCATGGTGATGTATGCCTTTCGTGCGGAACGCAAACACATTTCCGAAAGCGAAACCCA > W3110S.gb/2146249‑2146310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |