| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,302,408 | T→C | S169G (AGC→GGC) | napH ← | ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,302,408 | 0 | T | C | 100.0% | 17.4 / NA | 7 | S169G (AGC→GGC) | napH | ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/7); total (0/7) | |||||||||||
ACCAGGCTACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTA > W3110S.gb/2302401‑2302461 | aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:2629545/61‑1 (MQ=255)aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:2679101/61‑1 (MQ=255)aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:2808166/61‑1 (MQ=255)aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:2888581/61‑1 (MQ=255)aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:79690/61‑1 (MQ=255)aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:822108/61‑1 (MQ=255)aCCAGGCCACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTa < 1:935155/61‑1 (MQ=255) | ACCAGGCTACGCCCCATCAGAGAAACGGGGTTGATCCATTCCCAAATGAGCGTGCCAGTTA > W3110S.gb/2302401‑2302461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |