Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,308,799	C→A	E430D (GAG→GAT)	mqo ←	malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,308,799	0	C	A	100.0%	72.9 / NA	23	E430D (GAG→GAT)	mqo	malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/23);  total (0/23)

CACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAA  >  W3110S.gb/2308759‑2308818                                         |                    cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2371307/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:984694/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:740900/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:60246/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:353617/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2923187/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2795797/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2792523/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2729910/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2704091/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2699204/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2654869/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:1197062/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2332787/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2307951/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2250098/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2202338/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2096664/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:2010352/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:1915868/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:1703836/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:140549/60‑1 (MQ=255) cACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTATCGGCATCACGCTTGATaa  <  1:1215052/60‑1 (MQ=255)                                         |                    CACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAA  >  W3110S.gb/2308759‑2308818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑