Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 71,175 A→G R263R (CGA→CGG)  araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb71,1750AG100.0% 660.1 / NA 190R263R (CGA→CGG) araCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (83/107);  total (83/107)

Only 100 of 190 total aligned reads displayed.

TATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGA  >  W3110S.gb/71121‑71231
                                                      |                                                        
tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa                                                   <  1:1503740/62‑1 (MQ=255)
tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa                                                   <  1:2309112/62‑1 (MQ=255)
tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa                                                   <  1:1306382/62‑1 (MQ=255)
 aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1947516/62‑1 (MQ=255)
 aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1195578/62‑1 (MQ=255)
    gCCACCGTCGGTCTCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:1434205/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:1254622/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:1536384/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:298637/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:2433948/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:38786/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:2429662/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:693813/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:812503/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:1244321/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:896636/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:1820920/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:917747/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:931490/54‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1033171/59‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:2148682/59‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:2710890/59‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:2067945/59‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:907889/59‑1 (MQ=255)
    gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1050556/59‑1 (MQ=255)
    gCCACCGTCGGTCCCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa                                                       <  1:1464466/54‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:268964/57‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:344323/57‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:876759/57‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1211649/57‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:2289529/57‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1950743/57‑1 (MQ=255)
      cACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:2110481/57‑1 (MQ=255)
          gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:1732966/53‑1 (MQ=255)
          gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:2181435/53‑1 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCTGGTATTTAAAAAATGCAcc                                        >  1:2505104/1‑62 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa                                                  <  1:535153/52‑1 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:1996461/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:2896599/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:2213444/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:2763452/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:1235217/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:1555389/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:1873680/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc                                           >  1:174516/1‑59 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc                                        >  1:2811530/1‑62 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc                                        >  1:419561/1‑62 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc                                        >  1:2216361/1‑62 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc                                        >  1:739697/1‑62 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc                                        >  1:235077/1‑62 (MQ=255)
           tcggtcgCAATGTTGGTTTTGACGATCAACTCTAGTTCTCGCGGGTATTTAAAAAATGc                                           >  1:2855017/1‑59 (MQ=255)
                 gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGcc                                  >  1:1585589/1‑62 (MQ=255)
                 gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGcc                                  >  1:1017178/1‑62 (MQ=255)
                 gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGcc                                  >  1:46658/1‑62 (MQ=255)
                    aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc                               <  1:20144/62‑1 (MQ=255)
                    aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc                               <  1:144396/62‑1 (MQ=255)
                    aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc                               <  1:1979971/62‑1 (MQ=255)
                       ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGccc                             >  1:666559/1‑61 (MQ=255)
                       ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg                            >  1:2745069/1‑62 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:606182/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:2047670/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:1593720/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:2675477/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:1500331/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:1935179/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:1397089/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:1603905/62‑1 (MQ=255)
                         ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:481289/62‑1 (MQ=255)
                             ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag                      <  1:5613/62‑1 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag                      <  1:2597308/61‑1 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:855057/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:748824/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:1125228/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:2696701/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:2037876/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:2553947/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:2536729/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:2464066/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:1617416/1‑62 (MQ=255)
                              tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGt                     >  1:198285/1‑62 (MQ=255)
                                aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag                          <  1:1986808/55‑1 (MQ=255)
                                aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc                   >  1:2649870/1‑61 (MQ=255)
                                aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc                   >  1:503583/1‑61 (MQ=255)
                                aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc                   >  1:1218903/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:2824942/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:1103990/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:1154217/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:2194897/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:1282398/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:2388835/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:2421062/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:1671268/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:2562582/1‑61 (MQ=255)
                                            aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg        >  1:1577006/1‑61 (MQ=255)
                                                  cgcgGGTATTTAAAAAATGCACCGGGTCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga  <  1:132788/61‑1 (MQ=255)
                                                  cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga  <  1:1286133/61‑1 (MQ=255)
                                                  cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga  <  1:2256103/61‑1 (MQ=255)
                                                  cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga  <  1:2238737/61‑1 (MQ=255)
                                                  cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga  <  1:1869338/61‑1 (MQ=255)
                                                  cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga  <  1:991849/61‑1 (MQ=255)
                                                      |                                                        
TATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGA  >  W3110S.gb/71121‑71231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑