Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,381,618	G→A	A215A (GCC→GCT)	yfbB ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,381,618	0	G	A	100.0%	195.9 / NA	58	A215A (GCC→GCT)	yfbB	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/58);  total (0/58)

TGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAA‑TTTGCTGTCACGTTCACCACATAA  >  W3110S.gb/2381589‑2381650                              |                                  ttgCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:721670/60‑1 (MQ=255) tGGGAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:471252/62‑1 (MQ=255) tGGCAGTCGTCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1285420/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCTCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2192331/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:503153/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2230080/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2270350/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2277632/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2301291/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2334445/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2361323/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2424441/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2617479/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2797889/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2854189/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2901207/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:292065/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2189640/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:546478/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:576863/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:588574/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:603182/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:637532/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:695035/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:783317/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:829216/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:952398/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:97923/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:991696/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1637411/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1036851/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:112380/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1226190/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1233492/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1371461/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:140281/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1419957/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1423761/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1475440/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1539500/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1590999/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1601135/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1011893/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1679566/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1681615/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1689668/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1714265/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1768000/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1840791/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1869955/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1908751/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2016848/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2066903/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2143147/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2175953/62‑1 (MQ=255) tGGCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:2181450/62‑1 (MQ=255)  ggCAGTCGGCAGCCAGTTCCGCCGCCAGAGCGCGGAATTTTGCTGTCACGTTCACCACataa  <  1:843694/62‑1 (MQ=255)  ggCAGTCGGCAGCCAGTTCCGACGCCAGAGCGCGGAA‑TTTGCTGTCACGTTCACCACataa  <  1:1466338/61‑1 (MQ=255)                              |                                  TGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAA‑TTTGCTGTCACGTTCACCACATAA  >  W3110S.gb/2381589‑2381650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑