Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,389,320	T→C	N358S (AAC→AGC)	yfbK ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,389,320	0	T	C	100.0%	187.4 / NA	55	N358S (AAC→AGC)	yfbK	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/21);  total (34/21)

TTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTT  >  W3110S.gb/2389284‑2389377                                     |                                                          ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTc                                             >  1:2677195/1‑51 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:448919/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2220886/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2296566/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2312268/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2389709/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2776881/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2811330/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:324011/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:388983/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1196171/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:480377/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:745782/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:775466/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:862616/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:879792/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:960242/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:962408/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:972118/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1598375/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1009811/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1242135/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1274187/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1469736/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1556085/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1593714/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1621329/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:18727/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2129450/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:2100273/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaaa                                  >  1:1921341/1‑62 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaa                                   >  1:2181020/1‑61 (MQ=255) ttACCAACATCGGCAATTCGCACCATCATTGCCTCGCTGTAATTGCTATTCCCCACGCCaa                                   >  1:447148/1‑61 (MQ=255) ttACCAACATCGGCAATTCGCACAATCATTGCCTCGCTGTAATTGCTATTcc                                            >  1:2459262/1‑52 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:1876692/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:1201030/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:1421729/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:829843/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:816068/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:238030/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:1524071/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:720770/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:719231/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:551611/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:497012/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2487831/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:158488/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2391959/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2827002/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2825795/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:1612932/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:243710/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2744994/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2493537/61‑1 (MQ=255)                                  tCGCTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGtt  <  1:2490275/61‑1 (MQ=255)                                     |                                                          TTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTT  >  W3110S.gb/2389284‑2389377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑