Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 23,942 G→A G518S (GGC→AGC)  ileS → isoleucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb23,9420GA100.0% 469.1 / NA 142G518S (GGC→AGC) ileSisoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (108/34);  total (108/34)

Only 100 of 142 total aligned reads displayed.

GCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATG  >  W3110S.gb/23886‑23987
                                                        |                                             
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2557097/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2871828/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:310906/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1909057/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:431902/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2117989/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:522913/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:217986/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2729419/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2701574/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1714177/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1708695/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2242630/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1505393/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:259404/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1028444/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:944914/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1094215/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1158436/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:904434/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:893734/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1310156/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:873278/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:849223/1‑61 (MQ=255)
gcgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGAACCTCAGcg                                            >  1:1723956/1‑60 (MQ=255)
gcgTTGAAGGCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:2628759/1‑61 (MQ=255)
 cgTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGcga                                           >  1:1916380/1‑60 (MQ=255)
             tGGCATCCAGTCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1620704/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2694281/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2579523/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2766554/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2587080/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2122617/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2494936/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2608493/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:2841668/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1788111/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:55945/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1049787/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1235944/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1369932/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1407248/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1422758/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1648880/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:770081/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:371505/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:1761103/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:528296/62‑1 (MQ=255)
             tGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:458029/62‑1 (MQ=255)
              ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGt                             <  1:822189/61‑1 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGc                                     >  1:532445/1‑35 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:558929/1‑62 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:967661/1‑62 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:2779740/1‑62 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:1070903/1‑62 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:2538072/1‑62 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:2466874/1‑62 (MQ=255)
                                gATCTCGATGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGacac          >  1:2435917/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCTGACACATTGGATg  >  1:522144/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg  >  1:1483637/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg  >  1:2219237/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg  >  1:817184/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg  >  1:2188364/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg  >  1:586211/1‑62 (MQ=255)
                                        tGCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATg  >  1:2856150/1‑62 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1881266/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:127199/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:528696/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1087154/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1782049/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1756261/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1725106/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:709565/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1701044/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1524403/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:845884/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1338782/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:881904/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:883949/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1307053/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2718490/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2523743/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2568719/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2406344/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2382201/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2258993/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:264759/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2682762/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2228940/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2703988/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1852826/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2213663/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:2050456/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1980539/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1974366/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:373898/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGaa                      >  1:1857640/1‑41 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGa                       >  1:1959687/1‑40 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGCTGATCAGTACGTGa                       >  1:1833860/1‑40 (MQ=255)
                                         gCGAAAGAGATCCTCAGCGACGAAGATGATCAGTACGTGaa                      >  1:2055614/1‑41 (MQ=38)
                                                        |                                             
GCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATG  >  W3110S.gb/23886‑23987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑