| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,490,667 | T→C | F283S (TTT→TCT) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,490,667 | 0 | T | C | 100.0% | 21.3 / NA | 8 | F283S (TTT→TCT) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/0); total (8/0) | |||||||||||
GCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAC > W3110S.gb/2490644‑2490705 | gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGaaaaa > 1:1576732/1‑61 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:1825616/1‑62 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2064348/1‑62 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2321007/1‑62 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2577038/1‑62 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:2635018/1‑62 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAc > 1:606803/1‑62 (MQ=255)gCTTGATACAGGAAACCTGGCCTCTCTGAACAAAACATTAGAACTCACTGAACATGAAAAAc > 1:686193/1‑62 (MQ=255) | GCTTGATACAGGAAACCTGGCCTTTCTGAACAAACCATTAGAACTCACTGAACATGAAAAAC > W3110S.gb/2490644‑2490705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |