Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,499,989	A→G	intergenic (‑141/‑155)	yfdX ← / → ypdI	hypothetical protein/predicted lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,499,989	0	A	G	100.0%	335.1 / NA	97	intergenic (‑141/‑155)	yfdX/ypdI	hypothetical protein/predicted lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/64);  total (33/64)

TTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATACC  >  W3110S.gb/2499933‑2500037                                                         |                                                 ttttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1806677/62‑1 (MQ=255)  tttGTAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2213289/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTGTTTTAGTCTTGCAAtt                                             <  1:1612144/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:530510/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2029246/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1814913/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2715505/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2732702/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2825081/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1739439/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2830809/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1710275/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2054062/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:394981/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:458670/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1399937/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:122536/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1052844/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1037454/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1171213/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:893029/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1194522/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:815859/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1365000/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:7947/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:744169/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2159792/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1333873/61‑1 (MQ=255)  tttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATATTAGTCTTGCAAtt                                             <  1:1755343/61‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2618708/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:255781/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:990044/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2572891/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2401658/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2705970/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2865537/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:361298/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:529438/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:623222/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:672080/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:813021/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:988065/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1067227/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1290038/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1451905/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1508045/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:173626/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1749879/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:2056388/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1943239/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTCGCAAtt                                             <  1:27769/60‑1 (MQ=255)   ttGAAAGGGTTATCTTACAGTTGTAGTTATTATCCCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:1457228/60‑1 (MQ=255)                cTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAAtt                                             <  1:223057/47‑1 (MQ=255)                   aCAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTg                           >  1:2328581/1‑62 (MQ=255)                   aCAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTg                           >  1:3661/1‑62 (MQ=255)                   aCAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTg                           >  1:2331965/1‑62 (MQ=255)                   aCAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTg                           >  1:2696699/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTa                                          >  1:2085442/1‑46 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:949536/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1319361/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:6007/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1400439/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1286657/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1197272/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:846642/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:455246/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1507482/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:867727/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:320562/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:984103/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:2111798/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1745532/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:280720/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:2759175/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:98957/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:1752032/1‑62 (MQ=255)                    cAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGt                          >  1:2622768/1‑62 (MQ=255)                               attatCGCAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACt               <  1:1178612/62‑1 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCtt         >  1:2505988/1‑61 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCtt         >  1:2417130/1‑61 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:495879/1‑62 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:96704/1‑62 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:1079149/1‑62 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:1295443/1‑62 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:2397267/1‑62 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:372255/1‑62 (MQ=255)                                      cAAGAAGTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTta        >  1:1512015/1‑62 (MQ=255)                                            gcaTTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:2615801/60‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:1231565/62‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:522695/62‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:872973/62‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:361371/62‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:2883616/62‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:2878322/62‑1 (MQ=255)                                            gTATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:2288145/62‑1 (MQ=255)                                             tATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:456173/61‑1 (MQ=255)                                             tATTTTAGTCTTGCAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATAcc  <  1:2443043/61‑1 (MQ=255)                                                         |                                                 TTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAATATGCTTCCTGTCGCAGAGAACTATTCTTATATACC  >  W3110S.gb/2499933‑2500037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑