Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,503,886	T→C	intergenic (‑145/‑231)	yfdZ ← / → ypdA	prediected aminotransferase, PLP‑dependent/predicted sensory kinase in two‑component system with YpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,503,886	0	T	C	100.0%	110.2 / NA	33	intergenic (‑145/‑231)	yfdZ/ypdA	prediected aminotransferase, PLP‑dependent/predicted sensory kinase in two‑component system with YpdB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/33);  total (0/33)

CAGCGTTTTAGAACA‑TTTTATTAATGACGTTAAATGAGGAACTCTGCTTTAAAA  >  W3110S.gb/2503837‑2503890                                                   |     cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGTAACTCCACTTCaaaa  <  1:1945320/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2687651/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2291757/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2347196/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2367188/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2567960/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2593844/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2668177/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2678380/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1998568/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2716423/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:27776/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:341361/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:545148/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:591314/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:806399/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:882968/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:2285986/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1014570/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1986422/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1948703/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1896889/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1675131/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1638466/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1567578/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1389653/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1311141/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1300530/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1192213/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1172719/55‑1 (MQ=255) cAGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1083271/55‑1 (MQ=255) cAGCGTCTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1415873/55‑1 (MQ=255)  aGCGTTTTAGAACATTTTTATCAATGGCTTTAAATGAGGAACTCCACTTCaaaa  <  1:1888198/54‑1 (MQ=255)                                                   |     CAGCGTTTTAGAACA‑TTTTATTAATGACGTTAAATGAGGAACTCTGCTTTAAAA  >  W3110S.gb/2503837‑2503890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑