Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,505,539	A→G	K475E (AAA→GAA)	ypdA →	predicted sensory kinase in two‑component system with YpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,505,539	0	A	G	100.0%	84.9 / NA	26	K475E (AAA→GAA)	ypdA	predicted sensory kinase in two‑component system with YpdB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/5);  total (21/5)

CGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCG  >  W3110S.gb/2505517‑2505592                       |                                                      cGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATc                <  1:2525073/62‑1 (MQ=255) cGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATc                <  1:2243878/62‑1 (MQ=255) cGGTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATc                <  1:2826818/62‑1 (MQ=255)  ggTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATc                <  1:365979/61‑1 (MQ=255)   gTATTCAGCCTTGCAAAGGTGAAGGCGTTGTCACCATCAGCTTTGCAGAGTGCGGAAATc                <  1:725572/60‑1 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:2819810/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:2475812/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:2292939/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:2237163/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:1807389/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:332181/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt     >  1:559811/1‑62 (MQ=255)            cTTGCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAt      >  1:2108575/1‑61 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGTGGAAATCGGGTACGCATTGCg  >  1:29018/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGc   >  1:2820875/1‑61 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGc   >  1:1509059/1‑61 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1040928/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:752427/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:2301452/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1982671/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1811243/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1639193/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1607882/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1594788/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:15861/1‑62 (MQ=255)               gCAAAGGTGAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg  >  1:1195962/1‑62 (MQ=255)                       |                                                      CGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCG  >  W3110S.gb/2505517‑2505592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑