| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,517,999 | G→A | L52L (CTA→TTA) | mntH ← | manganese/divalent cation transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,517,999 | 0 | G | A | 98.6% | 225.6 / ‑4.6 | 69 | L52L (CTA→TTA) | mntH | manganese/divalent cation transporter |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (0/68); total (1/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATT > W3110S.gb/2517939‑2518032 | tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2846238/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1716023/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1777446/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1894064/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1920420/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1973233/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1997188/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1019447/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2457358/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2536504/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2622846/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2631600/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2729776/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:282294/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:2368061/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:433754/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:524594/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:536175/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:54304/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:581251/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:629113/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:841299/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:847324/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:867951/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:905700/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:912080/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:932651/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1705909/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1030020/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1129017/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1173155/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1207882/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1406329/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1479733/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:148836/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1619521/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1707070/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:1693716/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAg > 1:168402/1‑61 (MQ=255)tGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCAAAACTACAACCCACAGTAg > 1:334612/1‑61 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:459067/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2744344/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:444700/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:438397/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:429972/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:412352/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:334587/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:31023/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:287668/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1451697/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:146659/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1923402/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2749577/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:484513/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1401813/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1244804/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:5709/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:11838/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1182448/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:649743/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:814803/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:845421/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1720319/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1091949/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1088408/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1069352/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:913742/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2386235/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1647932/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1993070/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1611185/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:20220/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:20382/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2133168/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2136093/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:217451/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2209954/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2220761/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2293726/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2316994/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1892543/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2414398/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1522152/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2722705/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2680716/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2642002/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1521578/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:258585/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2558071/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2451302/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2475183/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2466262/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1574520/62‑1 (MQ=255) aGGTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCC‑CCTGAATAtt < 1:2485843/61‑1 (MQ=255) aGGTTAGCCCAAACGACAACCAACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1611136/62‑1 (MQ=255) ggTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCa > 1:2292540/1‑47 (MQ=255) ggTTAGCCCAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1741157/61‑1 (MQ=255) cAAACGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:2837517/53‑1 (MQ=255) aaaCGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:1844137/52‑1 (MQ=255) aaCGACAACCCACAATAACTGATAGCCGAAGCTGGCACCCGCCTGAATAtt < 1:807877/51‑1 (MQ=255) | TGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATT > W3110S.gb/2517939‑2518032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |