Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,607,203	T→A	F21I (TTC→ATC)	hyfG →	hydrogenase 4, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,607,203	0	T	A	100.0%	19.3 / NA	9	F21I (TTC→ATC)	hyfG	hydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/0);  total (9/0)

CGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTG  >  W3110S.gb/2607159‑2607220                                             |                  cGTCAAATCGTGTCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:2659937/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:155641/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:1574745/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:1639760/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:165685/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:2026694/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:2702160/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:581887/1‑62 (MQ=255) cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg  >  1:677285/1‑62 (MQ=255)                                             |                  CGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTG  >  W3110S.gb/2607159‑2607220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑