Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,674,071	C→T	G116D (GGC→GAC)	yphC ←	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,674,071	0	C	T	100.0%	61.0 / NA	20	G116D (GGC→GAC)	yphC	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/6);  total (14/6)

TCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGA  >  W3110S.gb/2674038‑2674126                                  |                                                        tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACTAGAAATAGGAAAACCGCGAc                              >  1:1146918/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:1179658/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:968311/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:919960/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:529487/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:525993/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:374070/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:291878/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:2751242/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:2742292/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:2197796/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:2151589/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:2079297/1‑61 (MQ=255) tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGTCAGTACAAGAAATAGGAAAACCGCGAc                              >  1:1799852/1‑61 (MQ=255)                             cTTCGTCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGa  <  1:2684945/61‑1 (MQ=255)                             cTTCGTCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGa  <  1:2493632/61‑1 (MQ=255)                             cTTCGTCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGa  <  1:850566/61‑1 (MQ=255)                             cTTCGTCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGa  <  1:1696950/61‑1 (MQ=255)                             cTTCGTCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGa  <  1:1657155/61‑1 (MQ=255)                              ttCGTCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGa  <  1:1890271/60‑1 (MQ=255)                                  |                                                        TCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGA  >  W3110S.gb/2674038‑2674126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑