| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,692,685 | A→T | A505A (GCT→GCA) | purL ← | phosphoribosylformyl‑glycineamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,692,685 | 0 | A | T | 88.5% | 145.5 / 9.9 | 52 | A505A (GCT→GCA) | purL | phosphoribosylformyl‑glycineamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base T (46/0); total (52/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
AGTTCCGGCATGGCGTTAGAAAGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCA > W3110S.gb/2692656‑2692735 | aGTTCCGGCATGGCGTTAGAAAGCCCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1903375/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAgg > 1:1922911/1‑54 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTgggct > 1:1614457/1‑60 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2848161/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2354677/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1054916/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2367708/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:25649/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2642048/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2648651/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2711485/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2717419/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2722426/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2741233/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2360379/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2877896/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:312167/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:325445/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:451477/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:666870/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:674976/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:714743/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:85182/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:906975/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:978034/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2323539/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1156104/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1289461/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1346068/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1364267/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1507094/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1543380/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1654461/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:180389/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:18251/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1910692/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2035160/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2328783/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2307826/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2066998/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2282376/1‑62 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:1678703/1‑61 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:551184/1‑61 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:675764/1‑61 (MQ=255)aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:1304923/1‑61 (MQ=255)aGTTCCGGCATGGCGTTAGAAACACCGCCTGCGCCAACGTCGTGTATAAACAGGAt > 1:636337/1‑56 (MQ=255) aaaGCCCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGcc > 1:2700543/1‑60 (MQ=255) aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTg > 1:2834512/1‑39 (MQ=255) aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:1089947/1‑61 (MQ=255) aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:51937/1‑61 (MQ=255) aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:1456159/1‑61 (MQ=255) aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:1719097/1‑61 (MQ=255) | AGTTCCGGCATGGCGTTAGAAAGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCA > W3110S.gb/2692656‑2692735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |