BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,820,171	G→A	A166V (GCA→GTA)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,820,171	0	G	A	100.0%	118.0 / NA	37	A166V (GCA→GTA)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (37/0); total (37/0)

CACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGG  >  W3110S.gb/2820123‑2820221

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTa                                                >  1:1661403/1‑53 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGgcgc                                          >  1:1800387/1‑59 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGTGCAcc                                       >  1:2239423/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1796542/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:2800416/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:2664911/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:258563/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:2896688/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:2442303/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:2902890/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1037725/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:2180971/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1938538/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1849398/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1761441/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1690807/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1522486/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:150720/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:580016/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCAcc                                       >  1:1353588/1‑62 (MQ=255)

cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATACCTATGGCGCAcc                                       >  1:363733/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:2200010/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:445474/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:62051/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:2864056/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:845824/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:919625/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:2777398/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:247877/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:1773115/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:1474882/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:1403633/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:1168960/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg  >  1:1108335/1‑62 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCgg   >  1:2581408/1‑61 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCgg   >  1:2307799/1‑61 (MQ=255)

                                     aGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCgg   >  1:1673825/1‑61 (MQ=255)

CACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGG  >  W3110S.gb/2820123‑2820221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`