| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,903,475 | C→T | L200L (TTG→TTA) | ygcF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,903,475 | 0 | C | T | 77.4% | 227.7 / 66.0 | 124 | L200L (TTG→TTA) | ygcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (28/0); new base T (0/96); total (28/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-28 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
ATTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGT > W3110S.gb/2903420‑2903514 | atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:1046928/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:2774919/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:1717403/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:2113199/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:383642/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:213251/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:262566/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:465481/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:1425065/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:47192/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:474246/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:516883/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:1379497/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:552302/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:874663/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:853920/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:1149380/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:1261444/1‑62 (MQ=255)atTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACgt > 1:2208315/1‑62 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGTAGt < 1:1937807/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2465383/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2602810/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2433210/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2667608/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2587065/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2576820/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2483361/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2704780/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1075355/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2737616/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:239623/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2782650/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2814901/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2818824/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2573114/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:400044/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:951375/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:803704/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:754396/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:603846/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:598409/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:55446/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:52344/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:478198/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:421097/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2887726/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:391346/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:390495/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:382825/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:377519/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:367590/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:356639/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2925398/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2916685/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2905860/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1381805/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1681526/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1636899/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:162238/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1611503/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1540046/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1539476/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1456579/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1414036/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1413155/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:138947/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2307193/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1356672/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1329627/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1262924/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1245674/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1212575/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1190781/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1180567/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1128403/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1109830/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1080820/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1735692/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2340849/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2275486/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2273366/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2262058/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2218263/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2205966/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2182933/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2166263/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2139695/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2081578/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2066881/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2020202/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:2004826/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1970029/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1902314/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1877137/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1828408/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1822176/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1819149/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:1715607/61‑1 (MQ=255) cgcAATGCAGGTATCGATACATAAACGCGTAGCATCTCCCTTCTGGCTAATTGGCTGAAGt < 1:194339/61‑1 (MQ=255) gcAATGCAGGTATCGATACATAAACGCGTAGCATCTTCCTTCTGGCTAATTGGCTGAAGt < 1:983730/60‑1 (MQ=255) | ATTTATGTGTTTGCATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGT > W3110S.gb/2903420‑2903514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |