Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 108,684 G→A A136T (GCG→ACG)  secA → preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb108,6840GA100.0% 28.0 / NA 10A136T (GCG→ACG) secApreprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/10);  total (0/10)

ACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTC  >  W3110S.gb/108679‑108740
     |                                                        
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTTGCCTGACTGTc  <  1:2392444/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:1036695/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:1155202/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:1684379/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:1845768/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:2000518/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:2780763/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:628326/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:631700/62‑1 (MQ=255)
aCCTGACGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTc  <  1:947460/62‑1 (MQ=255)
     |                                                        
ACCTGGCGCAACGTGACGCCGAAAACAACCGTCCGCTGTTTGAATTCCTTGGCCTGACTGTC  >  W3110S.gb/108679‑108740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑