| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,971,622 | A→G | F299F (TTT→TTC) | ygeD ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,971,622 | 0 | A | G | 99.1% | 355.9 / ‑4.9 | 105 | F299F (TTT→TTC) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (104/0); total (105/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
CCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCA > W3110S.gb/2971563‑2971662 | cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:2239184/62‑1 (MQ=255)cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:1116619/62‑1 (MQ=255)cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:424770/62‑1 (MQ=255)cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:74819/62‑1 (MQ=255)cccATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa < 1:631266/62‑1 (MQ=255) cATTACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:1603474/62‑1 (MQ=255) cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:488011/62‑1 (MQ=255) cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:2757818/62‑1 (MQ=255) cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaaaa < 1:2146771/62‑1 (MQ=255) cATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGAAGCTCGTGTTGCAGGGaaaaaa < 1:1120927/62‑1 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:305707/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1864173/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1963639/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:178145/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:21719/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1757968/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2209183/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1724240/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2252709/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2323133/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:2586659/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:96723/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:314813/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:359883/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:443774/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:597664/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:839956/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:922881/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1007972/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1093465/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1667670/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1303260/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1133932/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1156125/1‑49 (MQ=255) tCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGaaaa > 1:1332340/1‑49 (MQ=255) gCATCAACAAAGCATAGGCTGGCCGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2909360/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATc > 1:2740355/1‑50 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2732259/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2919176/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2906844/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2887865/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2797036/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:274501/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1287996/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2651624/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2647026/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2621838/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1352769/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1692578/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:296463/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1323819/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2579065/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:375815/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1269867/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1262895/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1238738/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:494613/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:502325/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:539403/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:546162/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1224383/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:789816/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:804431/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1030023/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:214312/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1697152/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1668101/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1762666/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1621264/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:159572/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:187563/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:190575/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1931860/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1961400/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1589289/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2032268/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2068313/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2073996/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2532691/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1554654/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:233646/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2527058/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:247932/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:241119/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2405612/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2370818/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2343810/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1543528/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1423526/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2269736/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2258779/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:146338/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:15002/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:153230/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:2195448/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacc > 1:471771/1‑56 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTAccaccaag > 1:1871627/1‑60 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTAccaccaag > 1:924669/1‑60 (MQ=255) gCATCAACAAAGCATAGGCTGGCAGCAGCTCGTGTTACAGGGAGAAAATCAGTacca > 1:2326583/1‑57 (MQ=255) gCATCAACAAAGCATAGGCTCGCAGCAGCTCGTGTTGCAGGGAGAAAATCAGTacca > 1:1071658/1‑57 (MQ=255) | CCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCA > W3110S.gb/2971563‑2971662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |