Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,973,286	G→A	T462I (ACC→ATC)	aas ←	fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,973,286	0	G	A	100.0%	117.2 / NA	36	T462I (ACC→ATC)	aas	fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/30);  total (6/30)

AGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAT  >  W3110S.gb/2973236‑2973335                                                   |                                                  aGAAGTCATTCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:2703831/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:1814351/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:2551551/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:2707148/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:2151029/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:2801151/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:2849177/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:1800729/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:398156/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:543640/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:1073581/62‑1 (MQ=255) aGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGaacaac                                        <  1:943531/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCTGAACAACACGGTGCaa                               <  1:661060/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1060840/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2871374/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2738666/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2715515/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:862878/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:87150/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2417954/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2138588/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2048123/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1999588/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1969277/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:2435060/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1841457/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1269216/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1659648/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCCGAACAACACGGTGCaa                               <  1:1572206/62‑1 (MQ=255)          aCGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGATGCC‑AACAACACGGTGCaa                               <  1:996644/61‑1 (MQ=255)                                       aGGAAAGTCGAGATGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAt  >  1:586933/1‑62 (MQ=255)                                       aGGAAAGTCGAGATGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAt  >  1:733171/1‑62 (MQ=255)                                       aGGAAAGTCGAGATGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAt  >  1:1368925/1‑62 (MQ=255)                                       aGGAAAGTCGAGATGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAt  >  1:1704420/1‑62 (MQ=255)                                       aGGAAAGTCGAGATGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAt  >  1:1005500/1‑62 (MQ=255)                                       aGGAAAGTCGAGATGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAt  >  1:2870181/1‑62 (MQ=255)                                                   |                                                  AGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACAACACGGTGCAACTGCGGTCATACACCAACTCCGGCACAAT  >  W3110S.gb/2973236‑2973335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑