Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	113,035	T→C	D62G (GAT→GGT)	coaE ←	dephospho‑CoA kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	113,035	0	T	C	100.0%	158.8 / NA	47	D62G (GAT→GGT)	coaE	dephospho‑CoA kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/7);  total (40/7)

TCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCA  >  W3110S.gb/112979‑113067                                                         |                                 tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:2130163/1‑61 (MQ=255) tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:2055857/1‑61 (MQ=255) tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:1941996/1‑61 (MQ=255) tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:2371774/1‑61 (MQ=255) tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:693968/1‑61 (MQ=255) tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:2873899/1‑61 (MQ=255) tctcTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcagc                              >  1:451941/1‑61 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:556745/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2001783/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1965637/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2552093/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1592326/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:708335/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:733037/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1227466/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1218074/1‑62 (MQ=255)      tCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1214637/1‑62 (MQ=255)            ttGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcag                               <  1:1531049/49‑1 (MQ=255)            ttGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcag                               <  1:1389475/49‑1 (MQ=255)            ttGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcag                               <  1:1027835/49‑1 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAgcagca                          >  1:2195960/1‑47 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCaa                         >  1:822266/1‑48 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCaa                         >  1:1882292/1‑48 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1970807/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1155048/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1371408/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1446482/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:706222/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:702904/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1525560/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:557049/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1722628/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:518926/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1788616/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2970/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2911299/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1813229/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2873663/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2397909/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:1925545/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:235820/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2246793/1‑49 (MQ=255)                   aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt                        >  1:2097406/1‑49 (MQ=255)                            cgcgCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAATCATGTTAGCGCCAAAGTGATCa  <  1:368914/62‑1 (MQ=255)                            cgcgCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAATCATGTTAGCGCCAAAGTGATCa  <  1:683773/62‑1 (MQ=255)                            cgcgCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAATCATGTTAGCGCCAAAGTGATCa  <  1:2588913/62‑1 (MQ=255)                            cgcgCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAATCATGTTAGCGCCAAAGTGATCa  <  1:715883/62‑1 (MQ=255)                                                         |                                 TCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCA  >  W3110S.gb/112979‑113067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑