Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,007,529	A→G	D37G (GAC→GGC)	ygeY →	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,007,529	0	A	G	100.0%	53.6 / NA	17	D37G (GAC→GGC)	ygeY	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/16);  total (1/16)

GATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGACGAGAAACGCGTAGTACAT  >  W3110S.gb/3007477‑3007548                                                     |                    gATATGACTTGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:2738517/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:1222598/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:1407605/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:1910816/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:1980030/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:2156166/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:679493/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:2214882/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:588079/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:2907066/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:32610/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:487197/62‑1 (MQ=255) gATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:362994/62‑1 (MQ=255)  atatGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:1243642/61‑1 (MQ=255)  atatGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:34134/61‑1 (MQ=255)            cTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAACGCGTAGTACAt  >  1:2214762/1‑61 (MQ=255)                       aCATGGTTGCTATTCCCAGTGAAAGCTGCGGCGAGAAAcg            <  1:2268272/40‑1 (MQ=255)                                                     |                    GATATGACTCGCTTCCTGCGCGACATGGTTGCTATTCCCAGTGAAAGCTGCGACGAGAAACGCGTAGTACAT  >  W3110S.gb/3007477‑3007548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑