Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,056,683 C→T P128P (CCG→CCA)  serA ← D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,056,6830CT83.0% 195.8 / 30.3 88P128P (CCG→CCA) serAD‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (6/9);  new base T (73/0);  total (79/9)
Fisher's exact test for biased strand distribution p-value = 8.76e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGT  >  W3110S.gb/3056652‑3056742
                               |                                                           
acacGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTc                                >  1:2290238/1‑61 (MQ=255)
acacGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTc                                >  1:2558289/1‑61 (MQ=255)
acacGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTc                                >  1:413242/1‑61 (MQ=255)
acacGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTc                                >  1:578916/1‑61 (MQ=255)
acacGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTc                                >  1:604080/1‑61 (MQ=255)
acacGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTc                                >  1:692126/1‑61 (MQ=255)
          gTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCa                     <  1:1637736/62‑1 (MQ=255)
               ctctAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:367920/4‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCTGCAGTTCGCCAATCACCAGCTc                 >  1:2564197/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGtt                                 >  1:1167539/1‑45 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCt                  >  1:405809/1‑60 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCt                  >  1:2762383/1‑60 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2917359/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:990381/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1019328/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2323727/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2347866/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2425346/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2458212/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:932199/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:917240/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2590211/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2704422/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2740134/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2741864/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:911570/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2800529/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2836950/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:635026/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:876449/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:331077/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:838012/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:385049/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:402375/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2298765/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:767729/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:544/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:54801/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:750405/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:586953/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:591908/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:601793/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:721442/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1248211/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1535422/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2284285/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1401779/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1384121/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1373363/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1355958/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1321514/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1292876/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1286720/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1443977/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1201732/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1194690/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:119324/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1146469/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1129122/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1125787/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1054765/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:104473/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1023400/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2044069/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2263956/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2237071/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2153609/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2135899/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2133436/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2065287/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1572214/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2030991/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1917322/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1763541/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1734580/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1703504/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1604591/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:157372/1‑61 (MQ=255)
               cTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGAAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:1443268/1‑61 (MQ=255)
               cTTTAGCATAGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTc                 >  1:2107061/1‑61 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:1883252/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:1325297/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:2569730/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:775739/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:2837685/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:844153/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGt  <  1:2160777/62‑1 (MQ=255)
                             tCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCACCGCAACAGAGCGCGt  <  1:982949/62‑1 (MQ=255)
                               |                                                           
ACACGCCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAATCACCAGCTCCGCAACAGAGCGCGT  >  W3110S.gb/3056652‑3056742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑