Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,120,719	A→C	V581V (GTT→GTG)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,120,719	0	A	C	89.4%	204.2 / 9.6	66	V581V (GTT→GTG)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (0/7);  new base C (0/59);  total (0/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCGGT  >  W3110S.gb/3120713‑3120775       |                                                         ttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:1016478/62‑1 (MQ=255) ttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:1762839/62‑1 (MQ=255) ttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:2644568/62‑1 (MQ=255) ttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:1449130/62‑1 (MQ=255) ttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:2749925/62‑1 (MQ=255) ttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:605793/62‑1 (MQ=255)  tCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgg   <  1:2211392/61‑1 (MQ=255)   cAGCCACCGGTATAGTCAGCAGATCGTCCAGCAGCTGTTCAAATTCAGCATTGAACTCgct  <  1:1243033/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCTGTTCAAATTCAGCATTGAACTCgct  <  1:41877/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2664003/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2160474/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2205481/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2116912/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2297384/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2369418/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2418740/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2422664/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2488945/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2521347/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2545477/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2633546/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:867296/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:729466/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2747207/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:866033/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:856202/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2839475/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:305373/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:326554/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:544937/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:596774/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:75791/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:695152/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:71053/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1010955/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1041988/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1046086/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1070376/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1122451/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1123622/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1169605/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1254081/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1270097/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1287470/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:133335/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1363969/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1370354/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1439589/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1441210/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1502734/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:2124901/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1817674/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1772256/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:174498/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1719726/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1709530/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1688942/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1662216/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:163454/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1587433/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1567590/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1533489/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1503019/61‑3 (MQ=255)   cAGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:1443002/61‑3 (MQ=255)    aGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:5131/60‑3 (MQ=255)    aGCCACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCgct  <  1:930787/60‑3 (MQ=255)       |                                                         TTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCGGT  >  W3110S.gb/3120713‑3120775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑