| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,192,980 | C→T | G487G (GGC→GGT) | yqiK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,192,980 | 0 | C | T | 90.0% | 123.7 / 5.2 | 50 | G487G (GGC→GGT) | yqiK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base T (0/45); total (0/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
TGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGC > W3110S.gb/3192928‑3193019 | tGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCg < 1:892582/62‑1 (MQ=255)tGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCg < 1:789873/62‑1 (MQ=255)tGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCg < 1:2251253/62‑1 (MQ=255)tGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCg < 1:29441/62‑1 (MQ=255)tGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCg < 1:2373145/62‑1 (MQ=255) cAGGTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTgggggc < 1:1969405/62‑2 (MQ=255) gggcGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2021171/58‑1 (MQ=255) ggTGGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2307232/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1001198/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2221971/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2229821/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2302457/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:237299/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2375896/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2410766/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2487621/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2699843/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2889059/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:373670/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:512423/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:531079/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:679932/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:687253/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:748688/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2080745/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1006917/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1047558/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1097584/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1103906/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1182095/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1239652/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1346343/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1416300/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1434721/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1520762/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1536451/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1556065/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1575608/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1811641/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1866035/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1878631/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1916515/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2199518/61‑1 (MQ=255) ggTCGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGGGGGGGGc < 1:27939/61‑1 (MQ=255) cGATGGGCTGAATCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1141657/58‑1 (MQ=255) tGGGCTGAATCTTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2651013/55‑1 (MQ=255) aTCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1570933/47‑1 (MQ=255) aTCGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2340040/47‑1 (MQ=255) tCGTGGTGGCGCTACGGGGGATGCAAACACGGGGAATGTGGGGGGc < 1:677614/46‑1 (MQ=255) cGTGGTGGCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:2355642/45‑1 (MQ=255) tggtggCGCTACGGGTGATGCAAACACGGGGAATGTGGGGGGc < 1:1776202/43‑1 (MQ=37) | TGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGC > W3110S.gb/3192928‑3193019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |