Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,196,615	C→T	A561A (GCG→GCA)	glnE ←	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,196,615	0	C	T	75.0%	76.8 / 25.1	48	A561A (GCG→GCA)	glnE	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (12/0);  new base T (36/0);  total (48/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGA  >  W3110S.gb/3196585‑3196655                               |                                         aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCgg                                      >  1:2266499/1‑35 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1017455/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:948273/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:273253/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:2462323/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:2098045/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:204643/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:2036828/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1605925/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1741556/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1702939/1‑62 (MQ=255) aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgc            >  1:908172/1‑61 (MQ=255)          ctggctggCACTCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:2187241/1‑61 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGTGAAATCAAATGATTAAGCGCCGCaggg   >  1:1023704/1‑61 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCaaa                     >  1:998837/1‑43 (MQ=38)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:1399192/1‑61 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1289816/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1074657/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1157224/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:878890/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:824736/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:775809/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:75502/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:742922/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:683741/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:340059/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2850533/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2733858/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1221715/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2720711/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2463106/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1573206/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1785619/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1907146/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1942584/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1974605/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1988564/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1593783/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1683478/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2116526/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2159217/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2180462/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1389483/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1296348/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2276317/1‑62 (MQ=255)          ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2389694/1‑62 (MQ=255)          ctggctggCAATCATCCGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:1202609/1‑61 (MQ=255)          ctggctggCAATCATCCGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:808479/1‑62 (MQ=255)                               |                                         ACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGA  >  W3110S.gb/3196585‑3196655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑