Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,196,615 C→T A561A (GCG→GCA glnE ← fused deadenylyltransferase and adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,196,6150CT75.0% 76.8 / 25.1 48A561A (GCG→GCAglnEfused deadenylyltransferase and adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (12/0);  new base T (36/0);  total (48/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGA  >  W3110S.gb/3196585‑3196655
                              |                                        
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCgg                                      >  1:2266499/1‑35 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1017455/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:948273/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:273253/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:2462323/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:2098045/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:204643/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:2036828/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1605925/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1741556/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgcg           >  1:1702939/1‑62 (MQ=255)
aCGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAgc            >  1:908172/1‑61 (MQ=255)
         ctggctggCACTCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:2187241/1‑61 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGTGAAATCAAATGATTAAGCGCCGCaggg   >  1:1023704/1‑61 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCaaa                     >  1:998837/1‑43 (MQ=38)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:1399192/1‑61 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1289816/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1074657/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1157224/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:878890/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:824736/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:775809/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:75502/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:742922/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:683741/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:340059/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2850533/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2733858/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1221715/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2720711/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2463106/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1573206/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1785619/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1907146/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1942584/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1974605/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1988564/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1593783/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1683478/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2116526/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2159217/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2180462/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1389483/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1296348/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2276317/1‑62 (MQ=255)
         ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2389694/1‑62 (MQ=255)
         ctggctggCAATCATCCGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:1202609/1‑61 (MQ=255)
         ctggctggCAATCATCCGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:808479/1‑62 (MQ=255)
                              |                                        
ACGCGCCAGCTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGA  >  W3110S.gb/3196585‑3196655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: