Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,196,651	G→A	P549P (CCC→CCT)	glnE ←	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,196,651	0	G	A	100.0%	99.2 / NA	31	P549P (CCC→CCT)	glnE	fused deadenylyltransferase and adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/0);  total (31/0)

CTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGA  >  W3110S.gb/3196594‑3196655                                                          |     ctggctggCACTCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:2187241/1‑61 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGTGAAATCAAATGATTAAGCGCCGCaggg   >  1:1023704/1‑61 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:1399192/1‑61 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2850533/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2180462/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2276317/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2389694/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2463106/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2720711/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2733858/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2116526/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:340059/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:683741/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:742922/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:75502/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:775809/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:824736/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:878890/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1573206/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1074657/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1157224/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1221715/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1289816/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1296348/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1389483/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:2159217/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1593783/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1683478/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1785619/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1907146/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1942584/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1974605/1‑62 (MQ=255) ctggctggCAATCATCGGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:1988564/1‑62 (MQ=255) ctggctggCAATCATCCGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCaggg   >  1:1202609/1‑61 (MQ=255) ctggctggCAATCATCCGCGATGCAGCACACAGAGAAATCAAATGATTAAGCGCCGCAGGGa  >  1:808479/1‑62 (MQ=255)                                                          |     CTGGCTGGCAATCATCGGCGACGCGGCACACAGAGAAATCAAATGTTTAAGCGCCGCGGGGA  >  W3110S.gb/3196594‑3196655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑