Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,199,980	C→T	P40S (CCG→TCG)	htrG →	predicted signal transduction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,199,980	0	C	T	100.0%	119.3 / NA	36	P40S (CCG→TCG)	htrG	predicted signal transduction protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/0);  total (36/0)

AGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAG  >  W3110S.gb/3199931‑3199986                                                  |       agaaCCGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2607517/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:359344/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2641737/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:266169/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2685948/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2820763/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2876055/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2886497/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:353380/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2627362/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:382666/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:426823/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:43677/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:503785/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:668771/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:691777/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:730134/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:731889/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1814269/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1071995/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1120739/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1281315/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1349168/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1564030/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1674850/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1743478/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1006467/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:1882046/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2089309/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2205127/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2269060/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:241343/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2430094/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGgag  >  1:2521706/1‑56 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGga   >  1:408380/1‑55 (MQ=255) agaaACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTTCGGga   >  1:2305251/1‑55 (MQ=255)                                                  |       AGAAACGCGCTATGTTTCCGACGAACTGAATACCTGGGTCCGCAGCGGTCCGGGAG  >  W3110S.gb/3199931‑3199986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑