Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,233,407	T→C	G375G (GGA→GGG)	ygjO ←	predicted methyltransferase small domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,233,407	0	T	C	100.0%	67.7 / NA	21	G375G (GGA→GGG)	ygjO	predicted methyltransferase small domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/0);  total (21/0)

GATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAA  >  W3110S.gb/3233354‑3233436                                                      |                              gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:129305/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:985464/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:677527/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:591265/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:1874604/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:1832435/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:1615336/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:1321525/1‑62 (MQ=255) gATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGCCCCAGCtt                       >  1:1315216/1‑62 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:1730831/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:2104824/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:2200825/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:2303722/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:2346190/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:2494641/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:2576633/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:634170/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:669465/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:945476/1‑35 (MQ=255)                                                 cgacgCCCCAGCTTCACCGCTTTCAGTACCACaaa  >  1:1190213/1‑35 (MQ=255)                                                 cgacgCCCCACCTTCACCGCTTTCAGTACCACaaa  >  1:811700/1‑35 (MQ=25)                                                      |                              GATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAA  >  W3110S.gb/3233354‑3233436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑