Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,233,462	C→T	C357Y (TGC→TAC)	ygjO ←	predicted methyltransferase small domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,233,462	0	C	T	100.0%	42.0 / NA	14	C357Y (TGC→TAC)	ygjO	predicted methyltransferase small domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/0);  total (14/0)

TTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTG  >  W3110S.gb/3233444‑3233505                   |                                            ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1234558/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1235903/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1361353/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1481877/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1774836/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1916160/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1918238/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:1925035/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:2575097/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:2886156/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:297448/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:595737/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg  >  1:69865/1‑62 (MQ=255) ttCGTCGCAATAGTGGTGTAGTAGCCGAAAAtcttct                           >  1:2423984/1‑37 (MQ=37)                   |                                            TTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTG  >  W3110S.gb/3233444‑3233505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑