Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,247,795	C→T	V57V (GTC→GTT)	yqjC →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,247,795	0	C	T	100.0%	200.2 / NA	61	V57V (GTC→GTT)	yqjC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/24);  total (37/24)

GCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAG  >  W3110S.gb/3247736‑3247852                                                            |                                                          gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2843543/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1652281/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1682733/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1686009/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2003777/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2081837/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2154092/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2301396/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2490040/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2689713/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:11092/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2875641/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2910417/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:570415/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:60250/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:640929/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:727079/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:757628/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:773294/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:927710/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1615364/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:113258/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1417623/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1597766/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1398197/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1254500/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1265650/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1289870/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1396285/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1483512/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1456696/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1367855/1‑62 (MQ=255) gCCGAAAAACACCAAAACCAGAATCGTATTGAAGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1312438/1‑62 (MQ=255)     aaaaaCACCAAAACC‑‑AATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:404487/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2909275/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2887569/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2597048/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1138744/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:768789/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1045937/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1658866/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1449083/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2265149/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2531778/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:217122/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1809637/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1530173/62‑1 (MQ=255)       aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGCGAAGTTCGGGCCaa                                                   <  1:1855105/62‑1 (MQ=255)        aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1386969/61‑1 (MQ=255)        aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:160745/61‑1 (MQ=255)        aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:455970/61‑1 (MQ=255)        aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1895426/61‑1 (MQ=255)        aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:72259/61‑1 (MQ=255)             cAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2216360/56‑1 (MQ=255)                                            aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaa               >  1:2676262/1‑61 (MQ=255)                                            aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaa               >  1:1569684/1‑61 (MQ=255)                                            aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaa               >  1:2676335/1‑61 (MQ=255)                                            aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCaga                >  1:201115/1‑60 (MQ=255)                                                        aaGTTCGGGGCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAg  <  1:164881/62‑1 (MQ=255)                                                        aaGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAg  <  1:410087/62‑1 (MQ=255)                                                        aaGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAg  <  1:2235846/62‑1 (MQ=255)                                                            |                                                          GCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAG  >  W3110S.gb/3247736‑3247852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑