Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,256,157	G→A	S149S (TCC→TCT)	yhaO ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,256,157	0	G	A	100.0%	26.4 / NA	10	S149S (TCC→TCT)	yhaO	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/0);  total (10/0)

AATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAT  >  W3110S.gb/3256134‑3256194                        |                                      aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGa      >  1:638573/1‑57 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACGAt  >  1:2865304/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCa   >  1:2349585/1‑60 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt  >  1:1000211/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt  >  1:1702381/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt  >  1:1807526/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt  >  1:1876324/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt  >  1:260808/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt  >  1:787757/1‑61 (MQ=255) aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACAAt  >  1:66384/1‑61 (MQ=255)                        |                                      AATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAT  >  W3110S.gb/3256134‑3256194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑