Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,393,749 T→C I789V (ATA→GTA)  yhdP ← conserved membrane protein, predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,393,7490TC92.0% 254.1 / 7.8 88I789V (ATA→GTA) yhdPconserved membrane protein, predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (0/7);  new base C (81/0);  total (81/7)
Fisher's exact test for biased strand distribution p-value = 1.58e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 131 total aligned reads displayed.

CACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCAC  >  W3110S.gb/3393720‑3393804
                             |                                                       
cacaTTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGAt                         <  1:1650359/62‑1 (MQ=255)
cacaTTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGAt                         <  1:271645/62‑1 (MQ=255)
cacaTTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGAt                         <  1:2847214/62‑1 (MQ=255)
      cTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGc                   <  1:563448/62‑1 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCATCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1041664/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTc                        >  1:198018/1‑40 (MQ=25)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAAt                     >  1:1029502/1‑43 (MQ=25)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACtt             >  1:1445926/1‑51 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTa           >  1:722558/1‑53 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCa        >  1:1032896/1‑56 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:183128/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:61612/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1811851/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1971465/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1775687/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:333891/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:608667/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2020781/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:605732/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2081421/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2126176/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:217684/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2202408/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:226625/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:469343/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2439102/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2510815/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:376455/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2593984/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2821741/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:282357/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:286207/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:2893872/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:308891/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:310133/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1765489/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1341524/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1237711/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1356169/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:835631/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1229992/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1166093/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:842949/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:139024/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:909231/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:953260/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:697822/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1018386/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1584379/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1592047/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:657771/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1624598/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1627514/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1658158/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1664774/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1672493/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1676764/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1753348/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:647965/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCcca   >  1:2046443/1‑61 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCcca   >  1:1775975/1‑61 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTACCATCccac  >  1:1287163/1‑61 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCAACTTTACCATCccac  >  1:635494/1‑62 (MQ=255)
                       gCTCTACGTTATACGTCGCGCCAGAATGATAAGGCAGCTCAATGCCCACTTTACCATCccac  >  1:1182128/1‑62 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:365734/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:335481/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:393922/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:422478/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:434951/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:816932/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:477511/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:711272/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:658747/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:953660/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1773387/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:109062/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:109670/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1121555/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1149725/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1358529/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1360518/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1474687/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1528821/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1533719/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1547304/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:160033/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1765336/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:329642/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:1887930/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2015020/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2067588/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2136798/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2209190/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2395574/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2426917/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2436845/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2523574/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2576249/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2674506/38‑1 (MQ=255)
                             tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg                    <  1:2695487/38‑1 (MQ=255)
                             |                                                       
CACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCAC  >  W3110S.gb/3393720‑3393804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: