Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,443,978	G→A	Q45Q (CAG→CAA)	thiS →	sulphur carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,443,978	0	G	A	79.2%	42.8 / 6.2	24	Q45Q (CAG→CAA)	thiS	sulphur carrier protein
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base A (0/19);  total (5/19)
Fisher's exact test for biased strand distribution p-value = 2.35e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCTTTT  >  W3110S.gb/3443919‑3444022                                                            |                                             gACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTg                                            >  1:1554999/1‑62 (MQ=255) gACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTg                                            >  1:1776615/1‑62 (MQ=255) gACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTg                                            >  1:2181029/1‑62 (MQ=255) gACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTg                                            >  1:2630225/1‑62 (MQ=255) gACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTg                                            >  1:2810993/1‑62 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:981975/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:119173/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:859659/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:728038/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:636158/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:599228/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:417364/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:408693/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:360933/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:2896624/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:2893084/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:2828176/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:2350652/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:1976775/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:1922685/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:1608643/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:1604272/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:1510813/62‑1 (MQ=255)                                           aTCGTCCCGCGGGAACAATGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCtttt  <  1:1212242/62‑1 (MQ=255)                                                            |                                             GACCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTGGGCGCAACATATCGTGCAGGATGGCGACCAGATCCTGCTTTT  >  W3110S.gb/3443919‑3444022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑