Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,473,263	T→C	intergenic (‑221/‑148)	btuB ← / → trmA	vitamin B12/cobalamin outer membrane transporter/tRNA (uracil‑5‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,473,263	0	T	C	100.0%	45.5 / NA	15	intergenic (‑221/‑148)	btuB/trmA	vitamin B12/cobalamin outer membrane transporter/tRNA (uracil‑5‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/0);  total (15/0)

CGCGTCAGCTCCAGATTCGCACTGGATTCCCTATTAACT  >  W3110S.gb/3473232‑3473270                                |        cgcgTCGGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2515625/1‑39 (MQ=38) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTaa    >  1:291429/1‑37 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAAc   >  1:1316287/1‑38 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:1133481/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:1881863/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:1895551/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2028334/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2214320/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2470771/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2616003/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2846803/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:2870790/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:458757/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:854714/1‑39 (MQ=255) cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt  >  1:941747/1‑39 (MQ=255)                                |        CGCGTCAGCTCCAGATTCGCACTGGATTCCCTATTAACT  >  W3110S.gb/3473232‑3473270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑